This means they display high selectivity compared with traditional ion exchangers. They are both based on the same multimodal anion exchange ligands and have similar ligand densities. Capto Adhere resin were purchased from GE Healthcare Life Sciences (Pittsburgh. Our Capto adhere and Capto adhere ImpRes resins can both be used for polishing in a two-step process in flow-through mode. This property could be used to isolate plasmid DNA from a crude Escherichia coli extract. Tricorn 5/20 and 5/50 columns, Capto MMC resin, Capto MMC ImpRes resin, and. Capto Adhere proved to offer a very strong binding of nucleic acids. One such example is Capto Adhere (Cytiva, Uppsala, Sweden). Capto Adhere is a recent multimodal chromatography material allowing molecular recognition between the ligand and target molecule, which is based on combined ionic and aromatic interactions. Multimodal anion exchanger for post-protein A purification of monoclonal antibodies. The small, high-resolution agarose beads coupled with selective multimodal ligands efficiently remove aggregates, viruses, host cell proteins and other contaminants in the MAb process, whilst retaining a high yield of MAb.Ĭapto MMC ImpRes uses a weak cation exchange ligand, effective in a broad pH/salt operational window, and is designed for use in the polishing step in MAb and domain antibody (DAb) purification.Ĭapto adhere ImpRes contains a strong anion exchange ligand for MAb polishing, and MAb charge variant separation, and is ideal for use after the initial capture step in either a two- or three-step purification. Q membrane capsule with Capto Q resin packed in conventional chromatography columns when. Capto adhere is a multimodal anion exchanger for intermediate purification and polishing of monoclonal antibodies after the Protein A capture step, giving the possibility to design a two-step chromatographic downstream process. Binding of xMuLV to Capto adhere appeared to tolerate higher levels of NaCl and impurity than QSFF, and showed some productspecific impact on binding that was not observed with QSFF. Capto adhere, a mixed mode chromatography resin with strong anion-exchange and reverse-phase interaction with solutes, was explored for purification of. Improved process economy is achieved through higher yields, reduced buffer consumption and the use of smaller columns and buffer tanks. The combination of high selectivity in binding and high resolution means greater load densities, smaller elution pool volume and improved impurity removal.
The slurry volume required for a desired column bed volume (volume of resin settled by gravity, V gs.
The slurry is calculated using the equation: 100 × (volume of drained resin - volume of frit)/10. Capto™ adhere ImpRes and Capto MMC ImpRes are highly-selective multimodal chromatography resins that enable high-resolution polishing of monoclonal antibodies (MAb) from the process development stage through to large-scale production.īoth resins utilize multiple types of interactions to offer significant advantages over traditional ion exchange resins, and the small bead size of the matrix gives excellent pressure/flow properties. The slurry can now be determined by the volume of drained resin remaining in the syringe minus the volume occupied by the frit ( 0.25 mL).
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